Several current measuring methods for activity of pyruvate dehydrogenase are compared and studied. 对目前几种丙酮酸脱氢酶测活的方法进行了比较研究。
Glucose oxidation via pyruvate dehydrogenase complex did not compensate for reduced palmitate oxidation rates. 通过丙酮酸脱氢酶复合物进行的葡萄糖氧化没有补偿棕榈酸酯氧化率的减少。
But2,6-DCPIP cant be used for measuring activity of pyruvate dehydrogenase complex, whereas CoA method is suitable with good sensitivity. 但2,6-二氯吲哚酚法不能用于测定丙酮酸脱氢酶复合体活性,这时用辅酶A法较好,其灵敏度也比较高。
The Cloning and Expression Optimization of Recombined Bacillus Thuringiensis Pyruvate Dehydrogenase 重组苏云金芽孢杆菌丙酮酸脱氢酶克隆及表达条件的优化
Methods Based on the principle of dehydrogenase coenzyme system, sodium pyruvate was used as a lactate dehydrogenase ( LDH) blocker, NAD+-NBT-Brij 98 was composed to enzyme coupling amplification technique in Tris HCl buffer. 方法基于脱氢酶辅酶体系的原理,用丙酮酸钠作乳酸脱氢酶(LDH)的阻止剂,在TrisHCl缓冲溶液中,由NAD+-NBT-Brij98组成酶偶联放大系统。
The activity of pyruvate carboxylase was increased as much as 40% when the medium was supplemented with Ca 2+. On the other hand, the activity of the pyruvate dehydrogenase complex was unaffected by the presence of Ca 2+. 当有Ca2+存在时,胞内丙酮酸羧化酶的活性最高可提高40%,而丙酮酸脱氢酶系的活性没有明显变化。
Intermittent peripheral weakness as the presenting feature of pyruvate dehydrogenase deficiency 以周期性外周肌无力为特征表现的丙酮酸脱氢酶缺乏症
Study on dynamic light scattering by photon correlation spectroscopy for pyruvate dehydrogenase 丙酮酸脱氢酶的光子相关动态光散射研究
Regulation of the Activity of the Mammalian Pyruvate Dehydrogenase Complex 哺乳动物中丙酮酸脱氢酶复合体的活性调节
E. coli Pp+ a carrying pPA was proved to have activity of pyruvate decarboxylase and higher activity of alcohol dehydrogenase than the original organism. 携带pPA的重组菌Pp+a被证明具有丙酮酸脱羧酶酶活且提高了乙醇脱氢酶酶活。
Cloning and expressing the E_2 subunit of pyruvate dehydrogenase complex 丙酮酸脱氢酶复合物E2亚单位的克隆表达与临床应用
Disorders of pyruvate dehydrogenase complex deficiency 丙酮酸脱氢酶复合物缺陷与疾病
Comparison of Measuring Methods for Activity of Pyruvate Dehydrogenase 丙酮酸脱氢酶测活方法比较研究
Especially new progresses in research of inhibitors of pyruvate dehydrogenase complex were introduced mainly. 主要介绍了具有除草活性好的丙酮酸脱氢酶抑制剂研究新进展。
Acetone; Fundamental studies on activity of pyruvate dehydrogenase 丙酮酸脱氢酶活性的基础研究
The production of astaxanthin can be improved from the genetic manipulation and fermentation control by enhance the activity of pyruvate dehydrogenase. 因此预计通过遗传改造和发酵控制提高丙酮酸脱氢酶的活性可能会进一步提高虾青素的产率。
Detection of anti-M2 autoantibody using recombinant pyruvate dehydrogenase complex E2 and its clinical significance 用重组丙酮酸脱氢酶复合物E2亚单位检测M2抗体及其临床意义
AMA in PBC are directed to the three ketoacid dehydrogenase complexes, and predominantly to E2 subunit of pyruvate dehydrogenase complex ( PDC-E2). PBC特异的AMA主要针对位于线粒体内膜的三个酮酸脱氢酶复合物家族(OADC)中的各个亚单位,即线粒体M2抗原的主要成分,其中最主要针对丙酮酸脱氢酶复合物E2亚单位(PDC-E2)。
Objective To develop an ELISA Kit by using prokaryotic expressed products for screening the autoantibodies to binding protein of E2 and E3 subunits ( E2BP and E3BP) of pyruvate dehydrogenase complex ( PDC) in the primary biliary cirrhosis ( PBC) patients. 目的用重组丙酮酸脱氢酶复合物E2亚单位和E3结合蛋白(PDCE2,PDCE3BP)建立筛查原发性胆汁性肝硬化(PBC)患者相应自身抗体的ELISA。
Objective To detect anti-M2 autoantibody using recombinant pyruvate dehydrogenase complex E2 ( PDC-E2) in order to help diagnosis of primary biliary cirrhosis ( PBC). 目的用重组表达的丙酮酸脱氢酶复合物E2亚单位(PDC-E2)检测M2抗体,以利于原发性胆汁性肝硬化(PBC)的早期诊断。
Objective To assay the primary biliary cirrhosis ( PBC) patients serum with the prokaryotic expression products of E2 subunit and E3 binding protein of pyruvate dehydrogenase complex. 目的建立一种利用重组表达的丙酮酸脱氢酶复合物E2亚单位(PDC-E2)和E3结合蛋白(PDC-E3BP)检测原发性胆汁肝硬变(PBC)患者血清的方法。
Objective To evaluate the measurement assay of magnesium ion in serum by a pyruvate kinase coupling lactate dehydrogenase and to observe the interference effect of several substance on the test of magnesium and serum magnesium level in patients with liver cirrhosis. 目的评价丙酮酸激酶偶联乳酸脱氢酶测定血清镁离子方法,观察几种物质对镁测定的干扰及肝硬化病人血清镁离子水平。
The increasing of the producing NO can directly makes the function of the complex I, complex II and pyruvate dehydrogenase in the respiratory chain decreased. NO生成增多可直接造成呼吸链中的复合体Ⅰ、复合体Ⅱ和丙酮酸脱氢酶等酶蛋白功能受损。
Objective To construct the expression vector of the pyruvate dehydrogenase complex E2 subunit gene ( PDC-E2). 目的构建丙酮酸脱氢酶复合物的E2亚单位(PDC-E2)的表达载体。
Third, Lactate and pyruvate ratio for red-eared turtle diving at low temperature is very stable in vivo may be closely related with high concentrations of lactate dehydrogenase and elevated calcium and magnesium ions concentration. 巴西龟乳酸与丙酮酸的比值在低温潜水期间非常稳定可能与体内高浓度乳酸脱氢酶和钙镁离子升高有关。
The pyruvate generate acetyl-CoA through the catalysis of pyruvate dehydrogenase, and acetyl-CoA is the precursor of fatty acid synthesis and ultimately into the fatty acid biosynthesis metabolism pathway. 丙酮酸经过丙酮酸脱氢酶系(乙酰辅酶A羧化酶)催化生成乙酰CoA,乙酰CoA是合成脂肪酸的前体物质,最终进入脂肪酸合成代谢途径。
The genes relate to energy metabolism are low abundance expression, mainly include fructose-bisphosphate aldolase, phosphoglycerate kinase, phosphoglucomutase, dihydrolipoamide dehydrogenase, pyruvate dehydrogenase, succinate dehydrogenase and so forth. 与能量代谢相关的基因主要有果糖二磷酸醛缩酶、磷酸甘油酸激酶、磷酸葡萄糖变位酶、二氢硫辛酰胺脱氢酶、丙酮酸脱氢酶、琥珀酸脱氢酶等低丰度表达的基因。
It was still found that DHA treatment for 24h induced the decrease of root activity, and increase activities and expression levels of pyruvate decarboxylase and alcohol dehydrogenase. 还发现DHA处理烟草成熟根系24h后的根系活力下降,同时丙酮酸脱羧酶和乙醇脱氢酶的活性和表达水平均升高。
The pyruvate oxidase mutation also resulted in the activation of glucose-6-phosphate dehydrogenase and acetyl-CoA synthetase. 丙酮酸氧化酶的缺陷同时也导致了6-磷酸葡萄糖脱氢酶和乙酰辅酶A合成酶活力的升高。
The deficiency of pyruvate oxidase did not cause the obvious accumulation of pyruvate, which indicated that under aerobic condition, the pyruvate metabolism mainly depends on the pyruvate dehydrogenase complex, and pyruvate oxidase does not play the key role. 丙酮酸氧化酶的缺失并没有导致显著的丙酮酸积累,从这一点我们可以看出,有氧条件下丙酮酸的代谢主要依赖于丙酮酸脱氢酶复合体,而丙酮酸氧化酶并不起决定作用。